Difference between revisions of "NGS"

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==Challenges==
 
==Challenges==
* Bad coverage of pharmacogenes. This most seriously affects WES.
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* Bad coverage of pharmacogenes. Notably, the lack of intronic variants in WES.
* Haplotype calling is challenging due to short read. NGS requires ''in silico'' haplotype estimation. There is support for basic haplotype estimation in [https://software.broadinstitute.org/gatk/documentation/tooldocs/3.8-0/org_broadinstitute_gatk_tools_walkers_haplotypecaller_HaplotypeCaller.php the GATK haplotype caller since version 3.3]. Haplotype calling can also be performed by various imputation methods [http://faculty.washington.edu/browning/beagle/beagle.html Beagle] or [http://dx.doi.org/10.1038/ng.3679 Eagle2] or [https://doi.org/10.1371/journal.pgen.1004234 SHAPEIT].
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* Haplotype calling is challenging due to short reads. NGS requires ''in silico'' haplotype estimation. There is support for basic haplotype estimation in [https://software.broadinstitute.org/gatk/documentation/tooldocs/3.8-0/org_broadinstitute_gatk_tools_walkers_haplotypecaller_HaplotypeCaller.php the GATK haplotype caller since version 3.3]. Haplotype calling can also be performed by various imputation methods [http://faculty.washington.edu/browning/beagle/beagle.html Beagle] or [http://dx.doi.org/10.1038/ng.3679 Eagle2] or [https://doi.org/10.1371/journal.pgen.1004234 SHAPEIT].
* Variants in homologous regions are hard to capture (regions with copy number variations (CNV)). Notably, the genes CYP2D6 and CYP2A6 are challenging. The CYP2D6 genotyping tool used by [https://github.com/PharmGKB/PharmCAT/wiki PharmCAT] is [https://www.nature.com/articles/npjgenmed201639 Astrolabe].
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* Difficult variant calling in homologous regions, such as CYP2D6. I.e. regions with copy number variations (CNV) or pseudogenes. The CYP2D6 genotyping tool that was used by [https://github.com/PharmGKB/PharmCAT/wiki PharmCAT] is [https://www.nature.com/articles/npjgenmed201639 Astrolabe]. Recently, a (seemingly) vastly improved program [https://github.com/inumanag/aldy Aldy], has been published.
* HLA-typing require special software. [https://doi.org/10.1002/cpt.411 Yang et al.] proposed to use [https://software.broadinstitute.org/cancer/cga/polysolver Polysolver] for whole exome sequencing (WES) or [https://github.com/FRED-2/OptiType OptiType] for whole genome sequencing (WGS). [https://doi.org/10.1101/356204 Reisberg et al.] proposed [https://doi.org/10.1371/journal.pone.0064683 SNP2HLA] for WGS.
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* HLA-typing require special software. There are many options. [https://doi.org/10.1002/cpt.411 Yang et al.] proposed to use [https://software.broadinstitute.org/cancer/cga/polysolver Polysolver] for whole exome sequencing (WES) or [https://github.com/FRED-2/OptiType OptiType] for whole genome sequencing (WGS). [https://doi.org/10.1101/356204 Reisberg et al.] proposed [https://doi.org/10.1371/journal.pone.0064683 SNP2HLA] for WGS. Recently, a (seemingly) vastly improved program [https://github.com/humanlongevity/HLA xHLA] has been published.  
 
* New variants are discovered, and needs to be [[gene function|functionally assessed]].
 
* New variants are discovered, and needs to be [[gene function|functionally assessed]].
  

Revision as of 10:37, 16 November 2018

Next Generation Sequencing (NGS) is an interesting technology for PGx

A nice overview of the Requirements for comprehensive pharmacogenetic genotyping platforms was published by Volker Lauschke et al. They claim that rare variants account for 30-40% of functional variability in PGx. However they argue that pre-emptive PGx should only include validated variants, and rare variants should be investigated only when the patient experience unexpected drug response.

Challenges

  • Bad coverage of pharmacogenes. Notably, the lack of intronic variants in WES.
  • Haplotype calling is challenging due to short reads. NGS requires in silico haplotype estimation. There is support for basic haplotype estimation in the GATK haplotype caller since version 3.3. Haplotype calling can also be performed by various imputation methods Beagle or Eagle2 or SHAPEIT.
  • Difficult variant calling in homologous regions, such as CYP2D6. I.e. regions with copy number variations (CNV) or pseudogenes. The CYP2D6 genotyping tool that was used by PharmCAT is Astrolabe. Recently, a (seemingly) vastly improved program Aldy, has been published.
  • HLA-typing require special software. There are many options. Yang et al. proposed to use Polysolver for whole exome sequencing (WES) or OptiType for whole genome sequencing (WGS). Reisberg et al. proposed SNP2HLA for WGS. Recently, a (seemingly) vastly improved program xHLA has been published.
  • New variants are discovered, and needs to be functionally assessed.

Solutions

Solutions for PGx on NGS data are given by Reisberg et al. in their article Translating genotype data of 44,000 biobank participants into clinical pharmacogenetic recommendations: challenges and solutions.

Interesting publications

Institution Article Comments
St. Jude Children’s Research Hospital Comparison of Genome Sequencing and Clinical Genotyping for Pharmacogenes WES and WGS can be used for PGx with in silico CNV calling and HLA calling
National Human Genome Research Institute Assessing the capability of massively parallel sequencing for opportunistic pharmacogenetic screening Suggests developing tools for PGx based on WES and WGS
University of Washington PGRNseq: A Targeted Capture Sequencing Panel for Pharmacogenetic Research and Implementation Targeted PGx panel with 84 genes
University of Tartu Translating genotype data of 44,000 biobank participants into clinical pharmacogenetic recommendations: challenges and solutions Well-described pipeline for PGx on biobank data. WES cannot be used for PGx (important variants missing, imputation and CNV calling difficult)