Pharmacolyzer

Pharmacolyzer - Personalizing the PGx recommendations

In order to get personalized medication recommendations for a patient that has been sequenced, the Pharmacolyzer does multiple queries to the Pharmacoracle.

The starting point is a JSON file with a representation of the patient VCF in Manchester syntax provided by the Pharmacotyper.

We have coined the verb to pharmacolyze for the action of the Pharmacolyzer

Sensitive data

Patient VCFs are sensitive data, and the VCF and Pharmacotyper data has to be stored on secure servers. It may also be sensible to run the pharmacolyzer in a facility for sensitive data, and only export the final pharmacolysis. Access to the final PGx analysis could be given through a REST API, and we describe one approach in the section below.

Examples of use of the Pharmacolyzer REST API

We have downloaded a VCF with samples from the 1000 genomes project. Four samples from the VCF was then pharmacotyped and stored in a JSON file called merged_patient.json. Pharmacolysis can be performed for the following drugs and patient samples:

Drugs	Samples
azathioprine clopidogrel	NA19461 NA19068 NA20754 NA21137

by running commands on the form

curl https://www.pgx.no/api/pharmacolyzer -d "patient_name=merged_patient&drugs=azathioprine&samples=NA19461&samples=NA21137"

from the command line. For pretty printing, the following can be used:

curl https://www.pgx.no/api/pharmacolyzer -d "patient_name=merged_patient&drugs=azathioprine&samples=NA19461"  | python -m json.tool